FastQCFastQC Report
Sun 20 Nov 2022
CO_003-COL.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCO_003-COL.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75745
Sequences flagged as poor quality0
Sequence length25-347
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC28553.769225691464783No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG28053.703214733645785No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG22743.002178361608027No Hit
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG21422.8279094329658725No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG21302.812066803089313No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC19762.608753053006799No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG19212.536140999405901No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA18372.425242590269985No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG14881.964486104693379No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT14271.8839527361542017No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG12011.585583206812331No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG11501.518252029836953No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC11211.4799656743019343No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT10891.4377186612977755No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG10571.3954716482936167No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG10351.3664268268532576No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA10341.3651066076968776No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG10341.3651066076968776No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA10301.359825731071358No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT10231.350584196976698No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT9861.3017360881906397No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT9661.2753317050630404No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT9521.256848636873721No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT9511.2555284177173411No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT9211.2159218430259422No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGC9091.2000792131493827No Hit
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT8981.1855568024292031No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT8951.1815961449600634No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA8871.1710343917090238No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG8641.1406693511122845No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG8591.134068255330385No Hit
GCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT8031.0601359825731071No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC7951.0495742293220673No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT7901.0429731335401677No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC7751.0231698461944683No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGG7560.998085682223249No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCG7290.9624397650009902No Hit
CCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT6970.9201927519968315No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCA6730.8885074922437125No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC6400.8449402600831738No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT6280.8290976302066143No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT6260.8264571918938544No Hit
CGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT6220.8211763152683346No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC6160.8132550003300548No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT6120.807974123704535No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT5380.710277906132418No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG5370.708957686976038No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT5130.6772724272229189No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT5080.6706713314410192No Hit
CCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT4660.6152221268730609No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGG4560.6020199353092613No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG4510.5954188395273615No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG4360.5756155521816622No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCA4150.547890949897683No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC4060.5360089774902633No Hit
GCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT4010.5294078817083636No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGC4000.5280876625519836No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAG3740.4937619644861047No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT3680.48584064954782497No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA3410.450194732325566No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA3390.4475542940128061No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT3340.44095319823090634No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG2940.38814443197570797No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAG2880.3802231170374282No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG2830.37362202125552846No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAG2800.36966136378638853No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT2470.3260941316258499No Hit
GGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT2430.32081325500033003No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT2430.32081325500033003No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAG2420.3194930358439501No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAG2320.3062908442801505No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCC2300.30365040596739057No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGC2160.28516733777807113No Hit
CTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT2140.2825268994653113No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAG1870.24688098224305235No Hit
GCCGCCTCCCTAATGTTACACACTGACTAG1850.24424054393029243No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCC1520.20067331176975375No Hit
GGCCGCCTCCCTAATGTTACACACTGACTAG1250.16502739454749488No Hit
TCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1140.15050498382731534No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGA1140.15050498382731534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)